It can. Technically, by having an I-130 filed on her behalf, there is a (small) measure of immigrant intent being displayed by your spouse. Such intent could be considered enough to have her refused entry in any status other than H or L, even tourist. But typically, since the chances that she will benefit from this I-130 are next to impossible (it will be at least 7 or 8 years before her case will can get GC) CBP let's these things go -- although they are not required to. Each crossing or renewal could see her denied entry. and if she is not Cdn, getting a consular visa may also be problematic.
Was there a particular reason why your in-laws made rather pointless , and in her case, harmful petition?